Week 17

From 2007.igem.org

Revision as of 10:19, 23 October 2007 by Francesca.bugane2 (Talk | contribs)
10/22/07
  • Ligation:
-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
  • Transformation:
-[http://partsregistry.org/Part:BBa_I763032 I763032] (on [http://partsregistry.org/Part:pSB4A3 pSB4A3]);
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763031 I763031] (on low copy plasmid), 3 different inocules using 3 different colonies;
-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (high copy plasmid).
-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).



10/23/07
  • Miniprep:
-[http://partsregistry.org/Part:BBa_I763040 I763040] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763029 I763029](high copy plasmid).
-[http://partsregistry.org/Part:BBa_I763029 I763029](low copy plasmid).
  • Control Digestion for results impagination:
-[http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763036 I763036];
-[http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763032 I763032];
-[http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763027 I763027];
-[http://partsregistry.org/Part:BBa_I763029 I763029] (low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763029 I763029] (high copy plasmid).
  • Inoculate [http://partsregistry.org/Part:BBa_I763032 I763032](on[http://partsregistry.org/Part:pSB4A3 pSB4A3]).



10/24/07



10/25/07



10/26/07



Back