Week 2

From 2007.igem.org

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We started plasmid amplification from IGEM 2007 plates.
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*We started plasmid amplification from IGEM 2007 plates.
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We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.  
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*We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.  
So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.
So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.
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*We also had some problems with Kanamycin. We first used it at a work concentration of  20 g/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly.
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*Preparation of IPTG stocks 100 mM (collegamento al protocollo)
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[[Bologna | Back]]
[[Bologna | Back]]

Revision as of 10:34, 6 August 2007

9/07/07 - 15/07/07


  • We started plasmid amplification from IGEM 2007 plates.
  • We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.

So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.

  • We also had some problems with Kanamycin. We first used it at a work concentration of 20 g/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly.
  • Preparation of IPTG stocks 100 mM (collegamento al protocollo)


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