Week 2

From 2007.igem.org

(Difference between revisions)
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*We started plasmid amplification from IGEM 2007 plates.
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*We start plasmid amplification from IGEM 2007 plates.
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*We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.  
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*We have some problems with Ampicillin solubility in water. We find that our Ampicillin was not soluble in water, so we get to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we try to change the antibiotic.  
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So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.
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So, we use a water- soluble Ampicillin with a concentration of 100 μg/ml. We find that it finally worked.
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*We also had some problems with Kanamycin. We first used it at a work concentration of  20 μg/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly.
+
*We also have some problems with Kanamycin. We first use it at a work concentration of  20 μg/ml, but it don’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it workes correctly.
*Preparation of [[Bologna_University/IPTG stocks preparation | IPTG stocks]] 100 mM
*Preparation of [[Bologna_University/IPTG stocks preparation | IPTG stocks]] 100 mM

Revision as of 09:30, 27 August 2007

07/09/07 - 07/15/07


  • We start plasmid amplification from IGEM 2007 plates.
  • We have some problems with Ampicillin solubility in water. We find that our Ampicillin was not soluble in water, so we get to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we try to change the antibiotic.

So, we use a water- soluble Ampicillin with a concentration of 100 μg/ml. We find that it finally worked.

  • We also have some problems with Kanamycin. We first use it at a work concentration of 20 μg/ml, but it don’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it workes correctly.


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