Week 2

From 2007.igem.org

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*We start plasmid amplification from IGEM 2007 plates.
*We start plasmid amplification from IGEM 2007 plates.
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*We have some problems with Ampicillin solubility in water. We find that our Ampicillin is not soluble in water, so we try to re-suspend it in NaOH 1M with work concentration of 50 μg/ml. Since bacterial cells still grow on our plates, we try to change the antibiotic.  
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*We have some problems with Ampicillin solubility in water. We find that our Ampicillin is not soluble in water, so we try to re-suspend it in NaOH 1M with working concentration of 50μg/ml. Since bacterial cells still grow on our plates, we try to change the antibiotic.  
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So, we use a water-soluble Ampicillin with work concentration of 100 μg/ml. We find that it finally worked.
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So, we use a water-soluble Ampicillin with working concentration of 100μg/ml. We find that it finally worked.
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*We also have some problems with Kanamycin. We first use it at work concentration of 20 μg/ml, but it doesn’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it works correctly.
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*We also have some problems with Kanamycin. We first use it at working concentration of 20μg/ml, but it doesn’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at working concentration of 50μg/ml and it works correctly.
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*Preparation of 100 mM of[[Bologna_University/IPTG stocks preparation | IPTG stocks]].
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*Preparation of 100mM of[[Bologna_University/IPTG stocks preparation | IPTG stocks]].
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[[Bologna | Back]]
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[[Bologna#Diary | Back]]

Latest revision as of 15:50, 26 October 2007

07/09/07 - 07/15/07


  • We start plasmid amplification from IGEM 2007 plates.
  • We have some problems with Ampicillin solubility in water. We find that our Ampicillin is not soluble in water, so we try to re-suspend it in NaOH 1M with working concentration of 50μg/ml. Since bacterial cells still grow on our plates, we try to change the antibiotic.

So, we use a water-soluble Ampicillin with working concentration of 100μg/ml. We find that it finally worked.

  • We also have some problems with Kanamycin. We first use it at working concentration of 20μg/ml, but it doesn’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at working concentration of 50μg/ml and it works correctly.


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