Week 2

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07/09/07 - 07/15/07


  • We started plasmid amplification from IGEM 2007 plates.
  • We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.

So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.

  • We also had some problems with Kanamycin. We first used it at a work concentration of 20 g/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly.


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