Week 3

From 2007.igem.org

(Difference between revisions)
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*We amplified some bio- bricks of interest for our project from the IGEM plates. We also amplified a plasmid (BBa_ J04430) to test the GFP fluorescence detection with our experimental set up.
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*We amplified some bio- bricks of interest for our project from the IGEM plates:
 +
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up
 +
-BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
 +
*We verified that the GFP (+LVA) displays...
 +
*BBa_J04500, the PLac promoter inducible by IPTG;
 +
*BBa_J04631, the GFP (+LVA) protein.  
::'''7/18/07'''
::'''7/18/07'''
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*We picked a colony in 5 ml of LB medium from each plate and incubated overnight
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*We picked a colony in 5 ml  
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*We picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.
*We picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.

Revision as of 13:23, 9 August 2007

07/17/07


  • We amplified some bio- bricks of interest for our project from the IGEM plates:
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up
-BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
  • We verified that the GFP (+LVA) displays...
  • BBa_J04500, the PLac promoter inducible by IPTG;
  • BBa_J04631, the GFP (+LVA) protein.


7/18/07
  • We picked a colony in 5 ml


  • We picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.


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