Week 3
From 2007.igem.org
(Difference between revisions)
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*We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed: | *We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed: | ||
| - | + | -BBa_ J04430 to test the GFP fluorescence detection with our experimental set up | |
| - | + | -BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project. | |
*We verified that the GFP (+LVA) displays... | *We verified that the GFP (+LVA) displays... | ||
*BBa_J04500, the PLac promoter inducible by IPTG; | *BBa_J04500, the PLac promoter inducible by IPTG; | ||
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::'''7/18/07''' | ::'''7/18/07''' | ||
| - | *We performed a picked a colony and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression. | + | *We performed a test for GFP induction with IPTG. We picked a colony from the J04430 plate and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression. |
*We picked a colony from 07/17 plates and inoculated in 5 ml of LB medium from each plate and incubated overnight. | *We picked a colony from 07/17 plates and inoculated in 5 ml of LB medium from each plate and incubated overnight. | ||
Revision as of 13:33, 9 August 2007
- 07/17/07
- We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:
-BBa_ J04430 to test the GFP fluorescence detection with our experimental set up -BBa_J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
- We verified that the GFP (+LVA) displays...
- BBa_J04500, the PLac promoter inducible by IPTG;
- BBa_J04631, the GFP (+LVA) protein.
- We straked on plates with the right antibiotic.
- 7/18/07
- We performed a test for GFP induction with IPTG. We picked a colony from the J04430 plate and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.
- We picked a colony from 07/17 plates and inoculated in 5 ml of LB medium from each plate and incubated overnight.
