Week 3

From 2007.igem.org

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*We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:  
*We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:  
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**J04430 to test the GFP fluorescence detection with our experimental set up
+
**J04430 to test the GFP fluorescence detection with our experimental set up;
-
**J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project.
+
**J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project;
**J04500, the PLac promoter inducible by IPTG;
**J04500, the PLac promoter inducible by IPTG;
**J04631, the GFP (+LVA) protein.  
**J04631, the GFP (+LVA) protein.  

Revision as of 13:30, 20 August 2007

07/17/07
  • We amplified some bio- bricks of interest for our project from the IGEM plates. We resuspended and transformed:
    • J04430 to test the GFP fluorescence detection with our experimental set up;
    • J04431 to test the GFP (+LVA) half-life since we needed a GFP with a short one for our project;
    • J04500, the PLac promoter inducible by IPTG;
    • J04631, the GFP (+LVA) protein.
  • We straked on plates with the right antibiotic.


07/18/07
  • We performed a test for GFP induction with IPTG. We picked a colony from the J04430 plate and grew it till a OD 0.4 – 0.6. We added 1 mM IPTG to induce GFP expression.
  • We picked a colony from 07/17 plates and inoculated in 5 ml of LB medium from each plate and incubated overnight.


07/19/07
  • We induced GFP expression from J04431 and J04430. Althought the first worked rigth in presence of IPTG, the second didn't. So, we performed a run on electroforesis gel for the J04430 plasmid. We found that it didn't match the rigth molecular weight. We thougth maybe there's something wrong with the plasmid.


07/20/07
  • Plasmid digestion (link):we digested J04500 with Spe1 and Pst1 and J04631 with Xba1 and Pst1.
  • We performed the gel extraction procedure and store at -20°C.
  • We amplified amd transformed I13507 and R0051 from the IGEM plate.


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