Week 8
From 2007.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
::'''08/21/07''' | ::'''08/21/07''' | ||
| - | *There isn't colonies for I763010 and C0012. we can't amplify this biobrick | + | *There isn't colonies for I763010 and C0012. we can't amplify this biobrick. |
| - | *J04631 digestion | + | *J04631 digestion. |
| - | * | + | *Band extraction. |
*R0051+P0412 (I763010)ligation. | *R0051+P0412 (I763010)ligation. | ||
| Line 14: | Line 14: | ||
::'''08/22/07''' | ::'''08/22/07''' | ||
| - | + | *There isn't any colony for R0051+P0412 (I763010) | |
::'''08/23/07''' | ::'''08/23/07''' | ||
| + | *A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul Ligasi). | ||
| + | *R0051, I763012 digestion with Spe/Pst1. | ||
| + | *P0412, J04631 digestion with Xba/Pst1. | ||
| + | *Run and extraction from gel. | ||
| + | *Trasformation of R0051+P0412 (I763010) ligations. | ||
Revision as of 13:36, 27 August 2007
- 08/20/07
- I763010 and C0012 trasformation.
- 08/21/07
- There isn't colonies for I763010 and C0012. we can't amplify this biobrick.
- J04631 digestion.
- Band extraction.
- R0051+P0412 (I763010)ligation.
- 08/22/07
- There isn't any colony for R0051+P0412 (I763010)
- 08/23/07
- A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul Ligasi).
- R0051, I763012 digestion with Spe/Pst1.
- P0412, J04631 digestion with Xba/Pst1.
- Run and extraction from gel.
- Trasformation of R0051+P0412 (I763010) ligations.
- 08/24/07
