Week 8

From 2007.igem.org

(Difference between revisions)
Line 23: Line 23:
*P0412, J04631 digestion with Xba/Pst1.
*P0412, J04631 digestion with Xba/Pst1.
*Run and extraction from gel.
*Run and extraction from gel.
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*Trasformation of R0051+P0412 (I763010) ligations.
+
*Transformation of R0051+P0412 (I763010) ligations.

Revision as of 13:39, 27 August 2007

08/20/07
  • I763010 and C0012 trasformation.


08/21/07
  • There isn't colonies for I763010 and C0012. we can't amplify this biobrick.
  • J04631 digestion.
  • Band extraction.
  • R0051+P0412 (I763010)ligation.



08/22/07
  • There isn't any colony for R0051+P0412 (I763010)


08/23/07
  • A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul Ligasi).
  • R0051, I763012 digestion with Spe/Pst1.
  • P0412, J04631 digestion with Xba/Pst1.
  • Run and extraction from gel.
  • Transformation of R0051+P0412 (I763010) ligations.



08/24/07
  • We plane new amplifications.


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