Week 8
From 2007.igem.org
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*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion. | *[http://partsregistry.org/Part:BBa_J04631 J04631] digestion. | ||
*Band extraction. | *Band extraction. | ||
| - | *[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])ligation. | + | *[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation. |
Revision as of 15:32, 3 September 2007
- 08/20/07
- 08/21/07
- There are no colonies for I763010 and C0012. We can't amplify these biobricks.
- J04631 digestion.
- Band extraction.
- R0051+P0412 (I763010) ligation.
- 08/22/07
- 08/23/07
- A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
- R0051, I763012 digestion with Spe/Pst1.
- P0412, J04631 digestion with Xba/Pst1.
- Run and extraction from gel.
- Transformation of R0051+P0412 (I763010) ligations.
- 08/24/07
- We plan new amplifications.
