Week 8

From 2007.igem.org

Revision as of 15:52, 26 October 2007 by Silvia.tamarri2 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

08/20/07

I763010 and C0012 transformation.

08/21/07

There are no colonies for I763010 and C0012. We can't amplify these biobricks.
J04631 digestion.
Band extraction.
R0051+P0412 (I763010) ligation.

08/22/07

There are no colonies for R0051+P0412 (I763010)

08/23/07

A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
R0051, I763012 digestion with Spe/Pst1.
P0412, J04631 digestion with Xba/Pst1.
Run and extraction from gel.
Transformation of R0051+P0412 (I763010) ligations.

08/24/07

We plan new amplifications.

Retrieved from "http://2007.igem.org/wiki/index.php/Week_8"