Week 9

From 2007.igem.org

(Difference between revisions)
Line 40: Line 40:
[http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sandbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)], [http://partsregistry.org/Part:BBa_E0020  E0020 ] and of [http://partsregistry.org/Part:BBa_R0040 R0040].
[http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sandbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)], [http://partsregistry.org/Part:BBa_E0020  E0020 ] and of [http://partsregistry.org/Part:BBa_R0040 R0040].
-
*[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] digestion with Spe/Pst1;
+
*Digestion for:
-
*[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] digestion with Xba/Pst1;
+
-[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] with Spe/Pst1;
-
*[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] digestion with Xba/Pst1;
+
 
-
*[http://partsregistry.org/Part:BBa_E0020 E0020] digestion with Xba/Pst1  
+
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
-
*[http://partsregistry.org/Part:BBa_R0040 R0040] digestion with Spe/Pst1  
+
 
 +
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Xba/Pst1;
 +
 
 +
-[http://partsregistry.org/Part:BBa_E0020 E0020] with Xba/Pst1 ;
 +
 
 +
-[http://partsregistry.org/Part:BBa_R0040 R0040] with Spe/Pst1.
*Control digestion for:
*Control digestion for:
-[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] with Eco/Pst1;
Line 50: Line 55:
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] with Eco/Pst1;
-
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1;
+
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1.
[[Bologna | Back]]
[[Bologna | Back]]

Revision as of 09:44, 6 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07

J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.

  • Digestion for:

-I763012 + S0103 with Spe/Pst1;

-B0034 + J22101 with Xba/Pst1;

-B0034 + J04631 (I763020) with Xba/Pst1;

-E0020 with Xba/Pst1 ;

-R0040 with Spe/Pst1.

  • Control digestion for:

-I763012 + S0103 with Eco/Pst1;

-B0034 + J22101 with Eco/Pst1;

-B0034 + J04631 (I763020) with Eco/Pst1.

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