Week 9

From 2007.igem.org

(Difference between revisions)
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::'''08/31/07'''
::'''08/31/07'''
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*Miniprep of [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] [http://partsregistry.org/Part:BBa_I763016 (I763016)], [http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox), [http://partsregistry.org/Part:BBa_B0034 B0034] +   
+
*Miniprep of :
-
[http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sandbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)], [http://partsregistry.org/Part:BBa_E0020  E0020 ] and of [http://partsregistry.org/Part:BBa_R0040 R0040].
+
-[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] [http://partsregistry.org/Part:BBa_I763016 (I763016)];
 +
 
 +
-[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox);
 +
 
 +
-[http://partsregistry.org/Part:BBa_B0034 B0034] +   
 +
[http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sandbox);
 +
 
 +
-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)];
 +
 
 +
-[http://partsregistry.org/Part:BBa_E0020  E0020 ];
 +
 +
-[http://partsregistry.org/Part:BBa_R0040 R0040].
*Digestion for:
*Digestion for:

Revision as of 09:46, 6 September 2007

08/27/07



08/28/07
  • Lab meeting on:

-model;

  • -LacI cloning under the pTetR R0040)promoter control.
08/29/07



08/30/07
  • There are colonies in the 07/30/07 plates.
  • We inoculate a E0020 and R0040 colony in 5ml.
  • We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).


08/31/07
  • Miniprep of :

-I763012 + J06550 (I763016);

-I763012+ S0103 (missed in the sandbox);

-B0034 + J22101 (missed in the sandbox);

-B0034 + J04631 (I763020);

-E0020 ;

-R0040.

  • Digestion for:

-I763012 + S0103 with Spe/Pst1;

-B0034 + J22101 with Xba/Pst1;

-B0034 + J04631 (I763020) with Xba/Pst1;

-E0020 with Xba/Pst1 ;

-R0040 with Spe/Pst1.

  • Control digestion for:

-I763012 + S0103 with Eco/Pst1;

-B0034 + J22101 with Eco/Pst1;

-B0034 + J04631 (I763020) with Eco/Pst1.

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