Week 9
From 2007.igem.org
(Difference between revisions)
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-[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1. | -[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)] with Eco/Pst1. | ||
| + | * Band extraction from gel for: | ||
| + | *Miniprep of : | ||
| + | -[http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] [http://partsregistry.org/Part:BBa_I763016 (I763016)]; | ||
| + | |||
| + | -[http://partsregistry.org/Part:BBa_I763012 I763012]+ [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sandbox); | ||
| + | |||
| + | -[http://partsregistry.org/Part:BBa_B0034 B0034] + | ||
| + | [http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sandbox); | ||
| + | |||
| + | -[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] [http://partsregistry.org/Part:BBa_I763020 (I763020)]; | ||
| + | |||
| + | -[http://partsregistry.org/Part:BBa_E0020 E0020 ]; | ||
| + | |||
| + | -[http://partsregistry.org/Part:BBa_R0040 R0040]. | ||
| + | |||
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 09:50, 6 September 2007
- 08/27/07
- New R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2ul Ligase).
- We transform R0051+P0412 (I763010) ligation and new S03520, S0100, B0034, J06550, S0103 biobricks.
- 08/28/07
- Lab meeting on:
-model;
- -LacI cloning under the pTetR R0040)promoter control.
- 08/29/07
- Miniprep of S03520, S0100, B0034, J06550, S0103.
- S03520, S0100, J06550, S0103 digestions with Xba/Pst1.
- B0034 digestion with Spe/Pst1.
- Run on electrophoresis gel and S03520, S0100, B0034 band extraction. We have some problems with J06550 plasmid vector due to its molecular weight (too heavy) and with S0103 insert for the same reason (too heavy). Thus, we decide to use another biobrick.
- Ligation for I763012 + J06550 (I763016), I763012 + S0103 (missed in the sendbox),B0034 + J22101 (missed in the sendbox), B0034 + J04631 (I763020).
- We transform the ligations.
- 08/30/07
- There are colonies in the 07/30/07 plates.
- We inoculate a E0020 and R0040 colony in 5ml.
- We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).
- 08/31/07
- Miniprep of :
-I763012+ S0103 (missed in the sandbox);
-B0034 + J22101 (missed in the sandbox);
-E0020 ;
-R0040.
- Digestion for:
-I763012 + S0103 with Spe/Pst1;
-B0034 + J22101 with Xba/Pst1;
-B0034 + J04631 (I763020) with Xba/Pst1;
-E0020 with Xba/Pst1 ;
-R0040 with Spe/Pst1.
- Control digestion for:
-I763012 + S0103 with Eco/Pst1;
-B0034 + J22101 with Eco/Pst1;
-B0034 + J04631 (I763020) with Eco/Pst1.
- Band extraction from gel for:
- Miniprep of :
-I763012+ S0103 (missed in the sandbox);
-B0034 + J22101 (missed in the sandbox);
-E0020 ;
-R0040.
