Week 9

From 2007.igem.org

(Difference between revisions)
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*[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1.
*[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1.
*Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We notice problems with [http://partsregistry.org/Part:BBa_J06550 J06550] band height (the insert is too heavy) and with [http://partsregistry.org/Part:BBa_S0103 S0103] band because the plasmid too heavy, however we extract insert bands for both.
*Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We notice problems with [http://partsregistry.org/Part:BBa_J06550 J06550] band height (the insert is too heavy) and with [http://partsregistry.org/Part:BBa_S0103 S0103] band because the plasmid too heavy, however we extract insert bands for both.
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*Ligation with a new protocol of I763012 + [http://partsregistry.org/Part:BBa_J06550 J06550] (I763016), I763012 + S0103 (missed in the sendbox),[http://partsregistry.org/Part:BBa_B0034 B0034] + J22101 (missed in the sendbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + J04631 (I763020).
+
*Ligation with a new protocol of [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] ([http://partsregistry.org/Part:BBa_I763016 I763016]), [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sendbox),[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sendbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763020 I763020]).
*We transform the ligations.
*We transform the ligations.

Revision as of 10:58, 31 August 2007

08/27/07



08/28/07
  • We hold a meeting and the topics of the day are:

-model agreed;

-we agree the position of LacI not controlled by Plac but by costitutionaly expressed as PTetR gene (R0040);

-reseach of genomic pLacI sequence to draw the primers or to order the synthesis.

  • We transform new biobricks:E0020 (to put after CMV J52034 promoter) and R0040 (whith witch adjust LacI expression);
  • We observe that there isn't a colony to R0051+P0412((I763010) ligation, so we abandon this way.
08/29/07



08/30/07
  • There are colonies in all ligations.
  • We inoculate a ligations, E0020 and R0040 colony in 5 ml.
  • Dimension tests with PMT to set gain and dimension area.


08/31/07

J22101 (missed in the sendbox), B0034 + J04631 (I763020), E0020 and of R0040.


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