Week 9
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* We inoculate a [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] colony in 5 ml; | * We inoculate a [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] colony in 5 ml; | ||
- | * | + | * There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763010 (I763010)] ligation. |
::'''08/29/07''' | ::'''08/29/07''' | ||
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*[http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] digestions with Xba/Pst1. | *[http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103] digestions with Xba/Pst1. | ||
*[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1. | *[http://partsregistry.org/Part:BBa_B0034 B0034] digestion with Spe/Pst1. | ||
- | *Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We | + | *Run on electroforesi gel and [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034] band extraction. We get some problems with [http://partsregistry.org/Part:BBa_J06550 J06550] plasmid vector molecular wheigh (too heavy) and with [http://partsregistry.org/Part:BBa_S0103 S0103] insert molecular weight (too heavy). So, we decide to use another biobrick. |
- | *Ligation | + | *Ligation for [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_J06550 J06550] ([http://partsregistry.org/Part:BBa_I763016 I763016]), [http://partsregistry.org/Part:BBa_I763012 I763012] + [http://partsregistry.org/Part:BBa_S0103 S0103] (missed in the sendbox),[http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J22101 J22101] (missed in the sendbox), [http://partsregistry.org/Part:BBa_B0034 B0034] + [http://partsregistry.org/Part:BBa_J04631 J04631] ([http://partsregistry.org/Part:BBa_I763020 I763020]). |
*We transform the ligations. | *We transform the ligations. | ||
Revision as of 15:35, 3 September 2007
- 08/27/07
- New R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2 ul Ligase).
- Transformation of R0051+P0412 (I763010) ligation and of new biobricks S03520, S0100, B0034, J06550, S0103.
- 08/28/07
- Lab meeting on:
-model -LacI cloning under the pTetR R0040)promoter control;
- 08/29/07
- Miniprep of S03520, S0100, B0034, J06550, S0103.
- S03520, S0100, J06550, S0103 digestions with Xba/Pst1.
- B0034 digestion with Spe/Pst1.
- Run on electroforesi gel and S03520, S0100, B0034 band extraction. We get some problems with J06550 plasmid vector molecular wheigh (too heavy) and with S0103 insert molecular weight (too heavy). So, we decide to use another biobrick.
- Ligation for I763012 + J06550 (I763016), I763012 + S0103 (missed in the sendbox),B0034 + J22101 (missed in the sendbox), B0034 + J04631 (I763020).
- We transform the ligations.
- 08/30/07
- There are colonies in the ligations.
- We inoculate ligations, E0020 and R0040 colony in 5 ml.
- We perform some tests to set up the fluorescence acquisition with the PMT (photomultiplier tube).
- 08/31/07
J22101 (missed in the sandbox), B0034 + J04631 (I763020), E0020 and of R0040.