Wisconsin/Agarose Gel

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(1.2% Agarose Gel preperation)
 
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===1.2% Agarose Gel preperation===
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===1.2% Agarose Gel preparation===
*8 wells
*8 wells
*600mg Agarose
*600mg Agarose
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*1uL 10mg/ml EtBr
*1uL 10mg/ml EtBr
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Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. Make sure everything is submerged in TBE before adding DNA.
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Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.  
 +
 
 +
*2uL DNA
 +
*12uL ddH<sub>2</sub>O
 +
*4uL 5x Loading Dye
 +
*4uL DNA Ladder for reference
 +
 
 +
Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.

Latest revision as of 19:49, 4 July 2007

1.2% Agarose Gel preparation

  • 8 wells
  • 600mg Agarose
  • 50mL TBE
  • 1uL 10mg/ml EtBr

Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.

  • 2uL DNA
  • 12uL ddH2O
  • 4uL 5x Loading Dye
  • 4uL DNA Ladder for reference

Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.