Wisconsin/Agarose Gel

From 2007.igem.org

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(1.2% Agarose Gel preparation)
 
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Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.  
Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.  
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*2uL DNA
*2uL DNA
*12uL ddH<sub>2</sub>O
*12uL ddH<sub>2</sub>O
-
*4uL 5x Dye
+
*4uL 5x Loading Dye
-
*DNA Ladder
+
*4uL DNA Ladder for reference
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Mix everything in tube before adding to well and ake sure gel box is submerged in TBE before adding DNA.
+
Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.

Latest revision as of 19:49, 4 July 2007

1.2% Agarose Gel preparation

  • 8 wells
  • 600mg Agarose
  • 50mL TBE
  • 1uL 10mg/ml EtBr

Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box.

  • 2uL DNA
  • 12uL ddH2O
  • 4uL 5x Loading Dye
  • 4uL DNA Ladder for reference

Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA.