Wisconsin/Digestion

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===Endonuclease Digestion===
===Endonuclease Digestion===
-
*10uL H2O
+
*10uL ddH<sub>2</sub>O
*5uL DNA
*5uL DNA
*2uL 10x buffer
*2uL 10x buffer

Latest revision as of 22:00, 4 July 2007

Endonuclease Digestion

  • 10uL ddH2O
  • 5uL DNA
  • 2uL 10x buffer
  • 2uL 1:10 dilution of BSA soln
  • 0.5uL Enzyme 1
  • 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use this website for restriction enzyme and this site for checking buffer compatibility.