Wisconsin/Digestion
From 2007.igem.org
< Wisconsin(Difference between revisions)
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===Endonuclease Digestion=== | ===Endonuclease Digestion=== | ||
- | *10uL | + | *10uL ddH<sub>2</sub>O |
*5uL DNA | *5uL DNA | ||
*2uL 10x buffer | *2uL 10x buffer |
Latest revision as of 22:00, 4 July 2007
Endonuclease Digestion
- 10uL ddH2O
- 5uL DNA
- 2uL 10x buffer
- 2uL 1:10 dilution of BSA soln
- 0.5uL Enzyme 1
- 0.5uL Enzyme 2
Incubate at 37C for 2-4 hours
Use this website for restriction enzyme and this site for checking buffer compatibility.