Wisconsin/Digestion

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Revision as of 21:59, 4 July 2007 by Klapoetke (Talk | contribs)

Endonuclease Digestion

  • 10uL H2O
  • 5uL DNA
  • 2uL 10x buffer
  • 2uL 1:10 dilution of BSA soln
  • 0.5uL Enzyme 1
  • 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use this website for restriction enzyme and this site for checking buffer compatibility.