Wisconsin/PCR
From 2007.igem.org
(Difference between revisions)
(→Basic Non-Sequencing PCR) |
|||
| Line 2: | Line 2: | ||
*38.5uL H<sub>2</sub>O | *38.5uL H<sub>2</sub>O | ||
*5uL 10x ExTaq buffer | *5uL 10x ExTaq buffer | ||
| + | *1uL 10mM dNTPs | ||
| + | *1uL Forward Primer | ||
| + | *1uL Reverse Primer | ||
| + | *1uL Template | ||
| + | *0.5uL ExTaq (add this last) | ||
| + | *2uL MgCl<sub>2</sub> 25mM | ||
| + | |||
| + | Run on Ansari PCR machine CDC-02 | ||
Revision as of 19:23, 4 July 2007
Basic Non-Sequencing PCR
- 38.5uL H2O
- 5uL 10x ExTaq buffer
- 1uL 10mM dNTPs
- 1uL Forward Primer
- 1uL Reverse Primer
- 1uL Template
- 0.5uL ExTaq (add this last)
- 2uL MgCl2 25mM
Run on Ansari PCR machine CDC-02
