Wisconsin/PCR
From 2007.igem.org
(Difference between revisions)
(→Basic Non-Sequencing PCR) |
|||
| Line 10: | Line 10: | ||
Run on Ansari PCR machine CDC-02 | Run on Ansari PCR machine CDC-02 | ||
| + | |||
| + | ===PCR Purification=== | ||
| + | Follow instruction in the [http://www1.qiagen.com/literature/protocols/QIAquickSpin.aspx QIAquick Spin Handbook] | ||
Revision as of 19:39, 4 July 2007
Basic Non-Sequencing PCR
- 38.5uL H2O
- 5uL 10x ExTaq buffer
- 1uL 10mM dNTPs
- 1uL Forward Primer
- 1uL Reverse Primer
- 1uL Template
- 0.5uL ExTaq (add this last)
- 2uL MgCl2 25mM
Run on Ansari PCR machine CDC-02
PCR Purification
Follow instruction in the QIAquick Spin Handbook
