Wisconsin/PCR

From 2007.igem.org

< Wisconsin(Difference between revisions)
 
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===Primer Stock===
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First check primer concentration using spectrophotometer... then prepare stock of 500uL 10uM.
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===Basic Non-Sequencing PCR===
===Basic Non-Sequencing PCR===
*38.5uL H<sub>2</sub>O
*38.5uL H<sub>2</sub>O

Latest revision as of 19:41, 4 July 2007

Primer Stock

First check primer concentration using spectrophotometer... then prepare stock of 500uL 10uM.

Basic Non-Sequencing PCR

  • 38.5uL H2O
  • 5uL 10x ExTaq buffer
  • 1uL 10mM dNTPs
  • 1uL Forward Primer
  • 1uL Reverse Primer
  • 1uL Template
  • 0.5uL ExTaq (add this last)
  • 2uL MgCl2 25mM

Run on Ansari PCR machine CDC-02

PCR Purification

Follow instruction in the QIAquick Spin Handbook