Wisconsin/PCR

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Basic Non-Sequencing PCR

38.5uL H₂O
5uL 10x ExTaq buffer
1uL 10mM dNTPs
1uL Forward Primer
1uL Reverse Primer
1uL Template
0.5uL ExTaq (add this last)
2uL MgCl₂ 25mM

Run on Ansari PCR machine CDC-02

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