Wisconsin/Protocol:Cell Culture

From 2007.igem.org

< Wisconsin(Difference between revisions)
 
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Using sterile technique:
Using sterile technique:
 +
1. Load 4mL of LB+Amp broth into culture tubes
1. Load 4mL of LB+Amp broth into culture tubes
 +
2. Use sterile stick and dab the desired bacterial colony from your plate
2. Use sterile stick and dab the desired bacterial colony from your plate
 +
3. Swirl stick in culture tube, ~5 sec.
3. Swirl stick in culture tube, ~5 sec.
 +
4. Flame culture tube before closing
4. Flame culture tube before closing
 +
5. Place culture tube in 37C shaker for 18-20 hrs.
5. Place culture tube in 37C shaker for 18-20 hrs.
 +
 +
Flame stick container and LB+Amp broth flask when done.
Flame stick container and LB+Amp broth flask when done.

Latest revision as of 17:29, 19 July 2007

Using sterile technique:

1. Load 4mL of LB+Amp broth into culture tubes

2. Use sterile stick and dab the desired bacterial colony from your plate

3. Swirl stick in culture tube, ~5 sec.

4. Flame culture tube before closing

5. Place culture tube in 37C shaker for 18-20 hrs.


Flame stick container and LB+Amp broth flask when done.