Wisconsin/Protocol:Ligation

From 2007.igem.org

< Wisconsin
Revision as of 16:24, 6 July 2007 by Klapoetke (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Ligation

Use Nanodrop to find vector and insert concentration in [ng/uL]. Find the length of vector and insert and calculate volume of reagents needed with this excel spreadsheet.

Heat inactivate ligation afterwards: 15 minutes at 65C.