NYMU Taipei/Lab Notes/2007 10 3

From 2007.igem.org

Contents

GEL check for digestions

System 1

  • TATA_INSA (X.P. digestion, 10/2)
  • TATB_INSB (E.S. digestion, 10/2)
  • CinR+HSL+D-term (E. digestion, 10/2)

System 2

  • OmpRBS (X. P. digestion, 10/2)

NYMU Taipei 20071003 TATA INSA,TATB INSB,OmpRBS,TATD IDE digestion check.jpg

  • lane1  : 1kd ladder
  • lane2-3: TATA_INSA (D. XP)
  • lane3-4: TATB_INSB (D. ES)
  • lane5-6: OmpRBS (D. XP)
  • lane7  : TATD_IDE (D. E)

three digestion for assembly

next tasks

  • pOmpC + TATA_INSA
  • TATB_INSB + CinR+HSL+D-term
  • pCinRHSL + OmpRBS