From 2007.igem.org

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• Applications:
  • Miniprep
  • Glycerol stocks

• Time to complete protocol:
  • Lab time: 15min
  • Waiting time:18hours
• Approximate cost of materials: $

Method from primary and secondary reagents

Primary & secondary Reagents Required including controls

• LB (cupboard)
• Selective Antibiotic (minus 20 freezer)
• Plate of transformed cells (cool room)

Method including controls

1. Aliquot 5mL LB into 50ml Falcon tube.
2. Add selective antibiotic in amount required for desired concentration.
   1. Add 10uL Ampicilin Stock (5mg/mL) leads to final concentration of 100ug/mL.
   2. Add 5uL Kanamycin Stock (5mg/mL) leads to final concentration of 50ug/mL.
3. Select single colony from Agar Plate and introduce to LB.
4. Incubate at 37degrees with shaking for approximately 18hrs (varies depending on growth rate of cells)

Equipement Required

• 50mL Falcon tubes
• Pipette and Tips
• 37degree shaker

References