From 2007.igem.org

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• Applications:
  1. Isolation of plasmid DNA from Bacterial cells

• Time to complete protocol:
  • Lab time: 1.5hrs
  • Waiting time: 10min
• Approximate cost of materials: $0.00

Method from primary and secondary reagents

Primary & secondary Reagents Required including controls

• Miniprep kit
• TAE Buffer if DNA intended for long term storage

Method including controls

• Use Protocol included in the Kit.
• A centrifugation method is used.
• Modifications:

In an effort to increase the yield of DNA, the following can be performed.

• • The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
  • The volume of bacterial culture used can be increased and centrifuged in two or more spins.

Equipement Required

• microcentrifuge: 1.7ml
• Pipettes and tips
• Microfuge

References

• Patricia Illing 03:49, 3 July 2007 (EDT):Miniprep protocol 3/July/2007