Paris/July 25

==Digestions==

Digestion of purifications of PCR products<br>

{|
|- style="background: #ccccff;"
! colspan="5" style="background: #ccccff;" | Digestion Products
|- style="background: #ccccff; text-align: center;"
|width=5%| Number
|width=20%| Product Name
|width=30%| Matrix Name
|width=5%| Enzyme 1
|width=5%| Enzyme 2
|width=5%| size
|width=35%| Description
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |D28
|RBS-DapAColi BB
|P8 (RBS-DapAColi)
|EcoRI
|PstI
|~950
|Biobrick
|
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |D29
|RBS-DapAColi
|P8 (RBS-DapAColi)
|XbaI
|PstI
|~950
|Backward insert
|
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |D30
|RBS-DapASubtilis BB
|P9 (RBS-DapASubtilis)
|EcoRI
|PstI
|~950
|Biobrick
|
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |D31
|RBS-DapASubtilis
|P9 (RBS-DapASubtilis)
|XbaI
|PstI
|~950
|Backward insert
|
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |D32
|Lox71-FtsA-FtsZ
|P10 Lox71-FtsA-FtsZ
|XbaI
|PstI
|~2600
|Backward insert - EcoRI site still presents : we can't use EcoRI
|
|
|}


== Ligation & transformation reactions ==


The Ligation reactions performed yesterday have all failed (the reactions were performed at 42° by mistake, then, another 1µL of ligase was added for a 5min rxn at 25°)

The following reactions were performed today

{|
|- style="background: #ccccff;"
! colspan="5" style="background: #ccccff;" | Ligations
|- style="background: #ccccff; text-align: center;"
|width=5%| Number
|width=25%| Insert
|width=5%| Insert Volume (µL)
|width=25%| Vector
|width=5%| Vector Volume (µL)
|width=35%| Comments
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L1
|D23.1 (AraC/pBad promoter FI)
|8.5
|D9.1 (J61002 ready for insertion of a FI)
|1.5
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L2
|D18 (BB dig. lox66DapAE.coli)
|7
|D22.1 (pSB1A2 Eco, Pst)
|3
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L3
|D19 (BB dig. lox66DapAsubtilis)
|7
|D22.1 (pSB1A2 Eco, Pst)
|3
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L4
|D20 (Lox66-DapAE.coli BI)
|8
|D1 (pJ23100 BV)
|2
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L5
|D21 (lox66-DapAsubtilis BI)
|6.5
|D1 (pJ23100 BV)
|2 (+1.5µL H2O)
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L6
|D14.1 (Cre ORF)
|8
|D15.1 (B0030 BV)
|2
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L7
|D27 (Lox71-ftsZ BI)
|6
|D1 (pJ23100 BV)
|2 (+2µL H2O)
|
|- style="background: #cccccc;"  
| style="background: #ccffcc;" |L8
|D27 (Lox71-ftsZ BI)
|6
|D3 (pJ23107 BV)
|2 (+2 H2O)
|
|}