PennState/Project/Dosimeter/Dosimetry

'''Fine Tuning'''<br>
The phage origins of the bio-dosimeter means that it is an all or nothing response on a cellular level. While this makes the dosimeter easy to use and interpret, it increases the challenge to set it up as a practical way to monitor ionizing radiation. The threshold at which the dosimeter triggers can be modified by testing various RBS sites in front of both cl and cro genes to change the rate of expression from the promoter. While changing the RBS strength will not have an affect on the maximum concentration of either protein as they are carefully regulated by the promoter, it will play a large role when the host's RecA protease from the SOS response begins cleaving Cl. Here the replacement rate of the two proteins will determine whether the on or off state is the end result of irradiation.


[[Image:Psuigemweakcrorbs.jpg|Thumb]
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a:hover.screenshot {
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<div class="podcast">
<!-- this is where you need to post the link to where it will go (the second one)-->
		<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
			Introduction
		</a></h3>
		<div class="screenshot">
	<!-- little white box thing -->		<a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter" 
			<img src="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg" width="133" height="100" alt="" /> 
			</a>
			<span class="download">
				<a href="http://openwetware.org/images/9/97/Psuigem2007promoterimage.jpg">Image Link</a>
			</span>
		</div>
	</div>

</div><div class="column">

<div class="podcast">
<!-- this is where you need to post the link to where it will go (the second one)-->
		<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/InitialTest','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
			Test Constructs
		</a></h3>
		<div class="screenshot">
	<!-- little white box thing -->		<a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/InitialTest" 
			<img src="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg" width="133" height="100" alt="" /> 
			</a>
			<span class="download">
				<a href="http://openwetware.org/images/b/be/Psuigem2007RBSimage.jpg">Image Link</a>
			</span>
		</div>
	</div>
</div>
<div class="column"><div class="podcast">
<!-- this is where you need to post the link to where it will go (the second one)-->
		<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/Dosimetry" ="window.popup_player_258600 = window.open('http://openwetware.org/images/c/c9/XylRdimer1.png','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
			Dosimetry
		</a></h3>
		<div class="screenshot">
	<!-- little white box thing -->		<a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/Dosimetry" 
			<img src="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg" width="133" height="100" alt="" /> 
			</a>
			<span class="download">
				<a href="http://openwetware.org/images/2/21/Psuigem2007geneimage.jpg">Image Link</a>
			</span>
		</div>
	</div>
</div>
<div class="column"><div class="podcast">
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		<h3><a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs" ="window.popup_player_258600 = window.open('https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs','post_258600','toolbar=no,scrollbars=no,directories=no,resizable=yes,width=360,height=305,top=20,left=20,location=no,menubar=no,status=yes,'); return false;">
			Final Constructs
		</a></h3>
		<div class="screenshot">
	<!-- little white box thing -->		<a rel="enclosure" href="https://2007.igem.org/PennState/Project/Dosimeter/FinalConstructs" 
			<img src="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg" width="133" height="100" alt="" /> 
			</a>
			<span class="download">
				<a href="http://openwetware.org/images/9/9b/Psuigem2007TTimage.jpg">Image Link</a>
			</span>
		</div>
	</div>

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