Wisconsin/PCR

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===Basic Non-Sequencing PCR===
*38.5uL H<sub>2</sub>O
*5uL 10x ExTaq buffer
*1uL 10mM dNTPs
*1uL Forward Primer
*1uL Reverse Primer
*1uL Template
*0.5uL ExTaq (add this last)
*2uL MgCl<sub>2</sub> 25mM

Run on Ansari PCR machine CDC-02

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