Berkeley Individual Contributions

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  <p><a href="https://2007.igem.org/Berkeley_UC">&lt;&lt;&lt; Return to UC Berkeley iGEM 2007 </a></p>
  <p><a href="https://2007.igem.org/BerkiGEM2007Present6">&lt;&lt;Previous Section: Human Practices</a> | <a href="https://2007.igem.org/BerkiGEM2007_Notebooks">Next Section: Team Notebooks&gt;&gt;</a></p>
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<h1 align="center">Individual Contributions</h1>
<p>The premise for this year's Berkeley iGEM project was based on the  winner of the "Bears Breaking Boundaries 2007 - Synthetic Biology  Contest." For this competition, undergraduate students submitted white  papers describing proposed applications of synthetic biology. The  essays were judged by a panel of faculty, postdocs, and graduate  students. The winning essay <em>Escherichia coli Based Red Blood Cell Substitutes: Cheap, High Volume, and Totally Awesome</em> was submitted by team member <strong>Austin Day</strong>. The project was then further  developed through discussions with the project's advisors. </p>
<p>The chassis for the project was built from genetic devices  constructed for the Tumor-Killing Bacteria project by <strong>Chris Anderson</strong>  and <strong>Austin Day</strong> in the <strong>Adam Arkin</strong> lab. A wbbL/neuS  constitutive-expression device that was specific to the the Bactoblood  project was constructed by team member <strong>Arthur Yu</strong>, and the resulting  strain was characterized by <strong>Samantha Liang</strong> and <strong>Austin Day</strong>. </p>
<p>The design for the controller came from discussions between  team member <strong>David Tulga</strong> and <strong>Chris Anderson</strong>. The R6K BAC plasmid, T7-GFP  reporter strain GH455G, and plasmid pBACr-AraGFP were developed  previously by <strong>Chris Anderson</strong>, but all other elements of the system were  cloned by the students. <strong>Arthur Yu</strong> identified, cloned, and characterized  the iron-responsive part. The BBb-format pSC101 plasmid, T7 promoter  variants, T7 RNA polymerase, and pir gene parts were cloned and  characterized by <strong>David Tulga</strong> as was the construction and tuning of the  RBS library for T7 RNA polymerase. Tuning of the RBS for the pir gene  was performed by <strong>David Tulga</strong> and <strong>Chris Anderson</strong>. </p>
<p>Cloning, construction, and characterization of the heme  biosynthesis cassette was performed by <strong>Kristin Doan</strong>. The hemoglobin,  AHSP, and other "chaperone" genes were synthesized as BBb basic parts  by DNA2.0 and Geneart. All composite parts containing these genes were  designed, constructed, and characterized by <strong>Austin Day</strong> and <strong>Vaibhavi  Umesh</strong>. The myoglobin and H-NOX were designed, constructed, and  characterized by <strong>Hannah Cole</strong>. </p>
<p>The kill switch was designed, constructed, and characterized by  <strong>Samantha Liang</strong>. The desiccation parts were designed, constructed, and  characterized by <strong>Vincent Parker</strong> and <strong>Nhu Nguyen</strong>. </p>
<p>The circuit diagrams, icons, and animated iGEM logo were  designed and constructed by <strong>David Tulga</strong>. The main page color scheme and  organization were designed in collaboration by <strong>Samantha Liang</strong> and <strong>David  Tulga</strong>. </p>
<p>The human practices component of our project was contributed by  <strong>Kristin Fuller</strong> under the guidance of <strong>Anthony Stavrianakis</strong>, <strong>Gaymon  Bennett</strong>, <strong>Paul Rabinow</strong>, <strong>Jennifer Saionz</strong>, and <strong>Maryanne McCormick</strong>. </p>
<p>Finally, all general lab duties (plate and gel pouring,  competent cell production, ordering) were performed by the students.  Day-to-day activities were supervised and guided by <strong>John Dueber</strong>, <strong>Chris  Anderson</strong>, <strong>Farnaz Nowroozi</strong>, <strong>Amin Hajimorad</strong>, and <strong>Rickey Bonds</strong> with  extensive administrative support provided by <strong>Kate Spohr</strong>, <strong>Kevin Costa</strong>,  and <strong>Gwyneth Terry</strong> <em>(thanks!)</em>. </p>
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<p align="center"><a href="https://2007.igem.org/Berkeley_UC">&lt;&lt;&lt; Return to UC Berkeley iGEM 2007 </a></p>
<p align="center"><a href="https://2007.igem.org/BerkiGEM2007Present6">&lt;&lt;Previous Section: Human Practices</a> | <a href="https://2007.igem.org/BerkiGEM2007_Notebooks">Next Section: Team Notebooks&gt;&gt;</a><a href="https://2007.igem.org/BerkiGEM2007_Resources"></a></p>
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