BerkiGEM2007-AustinConstructionFile38

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PCR AD0010F/AD0010R on I716072 (462 bp)<br>
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)<br>

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)<br>

Ligation product is I716088<br>

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