Paris/July 21
From 2007.igem.org
(Difference between revisions)
Eismoustique (Talk | contribs) (→Digestion reactions) |
Eismoustique (Talk | contribs) (→Digestion reactions) |
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| Line 53: | Line 53: | ||
|Cre ORF extracted for usage as a bw-insert | |Cre ORF extracted for usage as a bw-insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D15 |
|<bbpart>B0030</bbpart> bw-vector | |<bbpart>B0030</bbpart> bw-vector | ||
|<bbpart>B0030</bbpart> MP5.1 & MP5.2 | |<bbpart>B0030</bbpart> MP5.1 & MP5.2 | ||
| Line 60: | Line 60: | ||
|bacward vector for cloning a CDS bw-insert after the B0030 RBS | |bacward vector for cloning a CDS bw-insert after the B0030 RBS | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D16 |
|<bbpart>pSB1AK3</bbpart> vector | |<bbpart>pSB1AK3</bbpart> vector | ||
|<bbpart>B0015</bbpart> MP3.1 & MP3.2 | |<bbpart>B0015</bbpart> MP3.1 & MP3.2 | ||
| Line 67: | Line 67: | ||
|pSB1AK3 open vector for BioBrick cloning | |pSB1AK3 open vector for BioBrick cloning | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D17 |
|pSB1A2 <bbpart>E0422</bbpart> vector | |pSB1A2 <bbpart>E0422</bbpart> vector | ||
|<bbpart>E0422</bbpart> (MP6.1 & MP6.2) | |<bbpart>E0422</bbpart> (MP6.1 & MP6.2) | ||
| Line 74: | Line 74: | ||
|pSB1A2 open vector for BioBrick cloning | |pSB1A2 open vector for BioBrick cloning | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D18 |
|BB dig. lox66DapAE.coli | |BB dig. lox66DapAE.coli | ||
|lox66-DapAE.coli P5 PCR product | |lox66-DapAE.coli P5 PCR product | ||
| Line 81: | Line 81: | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D19 |
|BB dig. lox66DapAsubtilis | |BB dig. lox66DapAsubtilis | ||
|lox66-DapAsubtilis P6 PCR product | |lox66-DapAsubtilis P6 PCR product | ||
| Line 88: | Line 88: | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D20 |
|fw insert lox66-DapAE.coli | |fw insert lox66-DapAE.coli | ||
|lox66-DapAE.coli P5 PCR product | |lox66-DapAE.coli P5 PCR product | ||
| Line 95: | Line 95: | ||
|PCR product digested for usage as a forward insert | |PCR product digested for usage as a forward insert | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |D21 |
|fw insert lox66-DapAsubtilis | |fw insert lox66-DapAsubtilis | ||
|lox66-DapAsubtilis P6 PCR product | |lox66-DapAsubtilis P6 PCR product | ||
Revision as of 22:04, 21 July 2007
Digestion reactions
| Digestion Products | |||||
|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Description |
| D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | Vector with the J23100 promoter ready for insertion of a RBS/CDS backward-insert |
| D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | J23100 promoter ready for usage as a fw-insert |
| D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | Vector with the J23107 promoter ready for insertion of a RBS/CDS backward-inser |
| D12 | pJ23107 promoter fw-insert | pJ23100 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | J23107 promoter ready for usage as a fw-insert |
| D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | mRFP RBS/CDS/T sequence, can it be used as a backward inser? |
| D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | Cre ORF extracted for usage as a bw-insert |
| D15 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS |
| D16 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning |
| D17 | pSB1A2 E0422 vector | E0422 (MP6.1 & MP6.2) | EcoRI | PstI | pSB1A2 open vector for BioBrick cloning |
| D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick |
| D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick |
| D20 | fw insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert |
| D21 | fw insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert |
