Boston University/Plasmid Customization

From 2007.igem.org

(Difference between revisions)
(→Verifying the presence of the lacZ promoter in the plasmid pJQ200)
(→Excising the sacB gene from plasmid pJQ200)
Line 1: Line 1:
-
==Excising the sacB gene from plasmid pJQ200==
+
===Excising the sacB gene from plasmid pJQ200===
{| style="width:700pt;" border="1"
{| style="width:700pt;" border="1"
Line 28: Line 28:
|
|
:[[Image:BU_labeled_pjq200_sacb_digestion_7-17.jpg]]
:[[Image:BU_labeled_pjq200_sacb_digestion_7-17.jpg]]
 +
|}
 +
 +
===Verifying the presence of the lacZ promoter in the plasmid pJQ200===
 +
 +
{| style="width:700pt;" border="1"
 +
|
 +
:'''Double digestion of pJQ200 with EcoRI and HindIII'''
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
::::TEXT
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
|
 +
[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]]
 +
|
 +
[[Image:BU_labeled_lacz_PCR_7-21-2007.jpg]]
 +
|
 +
[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
|}
|}

Revision as of 18:18, 23 July 2007

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII





TEXT






BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

Retrieved from "http://2007.igem.org/wiki/index.php/Boston_University/Plasmid_Customization"