Boston University/Plasmid Customization

From 2007.igem.org

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(→Verifying the presence of the lacZ promoter in the plasmid pJQ200)
(→Excising the sacB gene from plasmid pJQ200)
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===Excising the sacB gene from plasmid pJQ200===
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'''Excising the sacB gene from plasmid pJQ200'''
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'''Verifying the presence of the lacZ promoter in the plasmid pJQ200'''
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:'''Double digestion of pJQ200 with EcoRI and HindIII'''
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::::TEXT
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[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]]
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[[Image:BU_labeled_lacz_PCR_7-21-2007.jpg]]
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[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
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Revision as of 18:19, 23 July 2007

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII





TEXT






BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

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