Paris/July 20
From 2007.igem.org
(Difference between revisions)
David.bikard (Talk | contribs) (→PCRs) |
David.bikard (Talk | contribs) |
||
| Line 17: | Line 17: | ||
|DNA= MP3_pSB1AK3-BBa_B0015 0.5µl | |DNA= MP3_pSB1AK3-BBa_B0015 0.5µl | ||
|Size= 1084 | |Size= 1084 | ||
| - | |Success= | + | |Success= ? |
|Image= | |Image= | ||
|Band= | |Band= | ||
| Line 37: | Line 37: | ||
|DNA= toothpick in MG1655 glycerol | |DNA= toothpick in MG1655 glycerol | ||
|Size= 2588 | |Size= 2588 | ||
| - | |Success= | + | |Success= ? The PCR seemed to have worked but the purification failed |
|Image= | |Image= | ||
|Band= | |Band= | ||
Revision as of 22:54, 23 July 2007
PCRs
| PCR : Lox71-KmR-Lox66 | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL | 1084 | |||
| 55 | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | 10 Lox71-KmR-F | 2.5µL | ? | ||
| 3m00' | Oligo R 10µM | 11 Lox66-KmR-R | 2.5µL | Image (click to enlarge) | ||
| Number cycles | Water | 34µl | [[Image:|30px]] | |||
| 35 | Polymerase | Phusion 0.5µL | Band (0=ladder) | |||
| DNA | MP3_pSB1AK3-BBa_B0015 0.5µl | |||||
| PCR : Lox71-FtsA-FtsZ | ||||||
|---|---|---|---|---|---|---|
| PCR Settings | Buffer (5x) | 5x 10µL | Expected size | |||
| Annealing (°C) | MgCl2 10µM | 10µM 0µL | 2588 | |||
| 55 | dNTP 10µM | 10µM 1µL | Success | |||
| Time Elongation | Oligo F 10µM | 3 Lox71-FtsA-F | 2.5µL | ? The PCR seemed to have worked but the purification failed | ||
| 3m00' | Oligo R 10µM | 2 FtsZ-R | 2.5µL | Image (click to enlarge) | ||
| Number cycles | Water | 34µl | [[Image:|30px]] | |||
| 35 | Polymerase | Phusion 0.5µL | Band (0=ladder) | |||
| DNA | toothpick in MG1655 glycerol | |||||
Glycerol stock
- BBa_pJ23107 clone 3&4 (S14)
- BBa_I0500 clone 1&2 (S13)
Minipreps on Biobricks
- BBa_pJ23107 clone 3&4
- BBa_I0500 clone 1&2
