BerkiGEM2007-AustinConstructionFile43

From 2007.igem.org

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Digest 9145-I716089 (Bgl2/Xho1/BsaI, 2574/1861/1088, Largest)<br>
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PCR AD0011F/AD0011R on I716073 (907bp)<br>
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Sub into any pTRC plasmid (Bgl2/Xho1, 4136/1713, Larger)<br>
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Digest this (Nde1/Bgl2, 858/38/11, Largest) <br>
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Product is I716093
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Digest I716089 (Nde1/Bgl2, 5815/8, Larger)<br>
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Ligation product is I716093<br>

Revision as of 06:13, 27 July 2007

PCR AD0011F/AD0011R on I716073 (907bp)
Digest this (Nde1/Bgl2, 858/38/11, Largest)

Digest I716089 (Nde1/Bgl2, 5815/8, Larger)

Ligation product is I716093

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