Paris/July 22
From 2007.igem.org
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elution of the purification products in 30-40microL H2O | elution of the purification products in 30-40microL H2O | ||
| - | == | + | == Overnight cultures launched == |
The following ON cultures were launched: | The following ON cultures were launched: | ||
| Line 15: | Line 15: | ||
I was unable to launch the following culture because of lack of a glycerol stock: | I was unable to launch the following culture because of lack of a glycerol stock: | ||
*pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep | *pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep | ||
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| + | == E.Coli pKS::DGAT == | ||
| + | |||
| + | We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See [[Paris/July_18#E.coli_pKS::DGAT|July 18]]). | ||
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| + | * Observation: | ||
| + | |||
| + | [[Image: Paris/coli_dgat_07222007|center|900px]] | ||
| + | |||
| + | |||
| + | * Interpretation: | ||
| + | |||
| + | We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences. | ||
Revision as of 15:38, 30 July 2007
Digestion products
Purification of the digestion products of 21/07/07 purif. using the SV wizard gel cleanup system
elution of the purification products in 30-40microL H2O
Overnight cultures launched
The following ON cultures were launched:
- pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep
- w121 (from glycerol stock S12) in 5 ml LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media
I was unable to launch the following culture because of lack of a glycerol stock:
- pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep
E.Coli pKS::DGAT
We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See July 18).
- Observation:
- Interpretation:
We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences.
