T7-RBS I716306
From 2007.igem.org
(Difference between revisions)
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| - | Biobrickificreation of T7 Promoters I716105 | + | Biobrickificreation of T7 Promoters I716105 <br> |
| - | + | PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)<br> | |
| - | PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A) | + | Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)<br> |
| - | Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) | + | Product A is pBca9145-I716105-PT7-wt <br> |
| - | Product A is pBca9145-I716105-PT7-wt | + | dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa <br> |
| - | + | Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L) <br> | |
| - | dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa | + | |
| - | + | ||
| - | Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L) | + | |
Produst is I716306 | Produst is I716306 | ||
Latest revision as of 21:14, 30 July 2007
Biobrickificreation of T7 Promoters I716105
PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt
dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
Produst is I716306
