Template:BerkiGEM2007 AustinAlcoholInformation
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==[[User:AustinDay|AustinDay]] 01:42, 31 July 2007 (EDT)== | ==[[User:AustinDay|AustinDay]] 01:42, 31 July 2007 (EDT)== | ||
* Can we create an injectable bacterium that can make you sober up quickly? I dunno, let's figure it out. | * Can we create an injectable bacterium that can make you sober up quickly? I dunno, let's figure it out. | ||
| - | * I think we want Class I alcohol dehydrogenases. | + | * I think we want Class I alcohol dehydrogenases. They have a lower Km, however there are many different polymorphisms of class I ADHs with different Kms, it appears as though having many different types would be the best/most biomimetic approach. |
* The first step catalyzed by the alcohol dehydrogenase is the limiting step right now. | * The first step catalyzed by the alcohol dehydrogenase is the limiting step right now. | ||
| - | * The second enzyme, acetaldehyde dehydrogenase will break down the very toxic acetaldehyde to acetic acid, which you pee out. | + | * The second enzyme, acetaldehyde dehydrogenase will break down the very toxic acetaldehyde to acetic acid, which you pee out. This is the pathway that ~90% of ethanol is metabolized by. |
* This might be a good place to use John's scaffolding technology to fuse the two enzymes together to reduce the concentration of the toxic intermediate? That'd be fun. | * This might be a good place to use John's scaffolding technology to fuse the two enzymes together to reduce the concentration of the toxic intermediate? That'd be fun. | ||
Revision as of 05:47, 31 July 2007
AustinDay 01:42, 31 July 2007 (EDT)
- Can we create an injectable bacterium that can make you sober up quickly? I dunno, let's figure it out.
- I think we want Class I alcohol dehydrogenases. They have a lower Km, however there are many different polymorphisms of class I ADHs with different Kms, it appears as though having many different types would be the best/most biomimetic approach.
- The first step catalyzed by the alcohol dehydrogenase is the limiting step right now.
- The second enzyme, acetaldehyde dehydrogenase will break down the very toxic acetaldehyde to acetic acid, which you pee out. This is the pathway that ~90% of ethanol is metabolized by.
- This might be a good place to use John's scaffolding technology to fuse the two enzymes together to reduce the concentration of the toxic intermediate? That'd be fun.
