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	==[[User:AustinDay|AustinDay]] 02:21, 31 July 2007 (EDT)==		==[[User:AustinDay|AustinDay]] 02:21, 31 July 2007 (EDT)==
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-	Enzyme-Catalyzed P-F Bond Hydrolysis of Monofluorophosphate as a Simple Model of Sarin Detoxification	+	This guy was using recombinant e coli to break down the very similar agricultural nerve agents. They used a mosquito esterase gene put into e. coli which expressed it on its surface. This leads me to the question of ... will sarin or other toxic nerve agents be able to pass through the cell membrane in order for the bacteria to break it down? How do I find out?
-	Norimasa Yoza1), Sachiko Nakashima1) and Tetsuya Nakazato1)	+
-	1) Department of Chemistry, Faculty of Science, Kyushu University	+
-	(Received September 17, 1996)	+
-		+
-	The catalytic ability of alkaline phosphatase (EC3.1.3.1) to promote P-F bond hydrolysis was examined with a simple nontoxic P-F compound, monofluorophosphate (Na2PO3F), to obtain an insight into the enzymatic degradation of Sarin, a toxic P-F compound. The enzyme recognized inorganic monofluorophosphate as a substrate and accelerated its hydrolytic conversion to orthophosphate and fluoride ions at pH 7.2 - 9.0 and 30 °C by a factor of ten billion (1010).	+

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Revision as of 06:30, 31 July 2007

AustinDay 02:21, 31 July 2007 (EDT)

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This guy was using recombinant e coli to break down the very similar agricultural nerve agents. They used a mosquito esterase gene put into e. coli which expressed it on its surface. This leads me to the question of ... will sarin or other toxic nerve agents be able to pass through the cell membrane in order for the bacteria to break it down? How do I find out?