Paris/August 3
From 2007.igem.org
(Difference between revisions)
(→DGAT biobrick: digestion, cloning) |
(→DGAT biobrick: digestion, cloning) |
||
Line 1: | Line 1: | ||
== DGAT biobrick: digestion, cloning == | == DGAT biobrick: digestion, cloning == | ||
- | ''' | + | *'''EcoR1-Pst1 digestion''' |
[[Image: DGATbbdigEcoR1Pst108032007.jpg|100px|right]] | [[Image: DGATbbdigEcoR1Pst108032007.jpg|100px|right]] |
Revision as of 14:39, 3 August 2007
DGAT biobrick: digestion, cloning
- EcoR1-Pst1 digestion
The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).