Paris/August 3
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== TOPO cloning == | == TOPO cloning == | ||
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| + | Cloning using TOPO protocol of the following purification of PCR products<br> | ||
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Revision as of 16:00, 3 August 2007
DGAT biobrick: digestion, cloning
- EcoR1-Pst1 digestion
The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).
TOPO cloning
Cloning using TOPO protocol of the following purification of PCR products
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments |
| P6 | Lox66-DapASubtilis | O8 Lox66-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 979 | |
| P7 | Lox71-FtsZ | O1 Lox71-FtsZ-F | O2 FtsZ-R | MG1655 | 1260 | EcoRI site in FtsZ not mutated |
| P8 | RBS-DapAColi | O20 RBS-DapAColi-F | O7 DapAColi-R | MG1655 | 952 | B0030-DapAColi |
