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		+	==[[User:AustinDay|AustinDay]] 21:14, 2 August 2007 (EDT)==
		+	* Chris said that if we can find a transporter that will pump in the toxin, that it might still work. I've started to spend a bit of time looking for some sort of organophosphate transporter in a bacterium. But I'll concentrate on the alcohol side trip more for now, I think it gets across the point that we can detox stuff using this e. coli chassis, and it avoids using a transporter.
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	==[[User:AustinDay|AustinDay]] 02:21, 31 July 2007 (EDT)==		==[[User:AustinDay|AustinDay]] 02:21, 31 July 2007 (EDT)==
-	*<br>	+	* All nerve agents belong to the organophosphorus (OP) compound group.
-	Enzyme-Catalyzed P-F Bond Hydrolysis of Monofluorophosphate as a Simple Model of Sarin Detoxification	+	* They inhibit acetocholinesterase, resulting in the buildup of acetocholine, which prevents muscles from relaxing.
-	Norimasa Yoza1), Sachiko Nakashima1) and Tetsuya Nakazato1)	+	* This other dude (I have all the refs on my computer, the wiki isn't very good for this) Has identified various phosphotriesterases that will detoxify a variety of organophosphates (pesticides, as well as the chemical weapons sarin and soman). He describes various mutants and their effects on the stereoselectivity.
-	1) Department of Chemistry, Faculty of Science, Kyushu University	+	* I've heard of two things used to detox:
-	(Received September 17, 1996)	+	** Phosphotriesterase
		+	** organophosphorus hydrolase (OPH)
-	The catalytic ability of alkaline phosphatase (EC3.1.3.1) to promote P-F bond hydrolysis was examined with a simple nontoxic P-F compound, monofluorophosphate (Na2PO3F), to obtain an insight into the enzymatic degradation of Sarin, a toxic P-F compound. The enzyme recognized inorganic monofluorophosphate as a substrate and accelerated its hydrolytic conversion to orthophosphate and fluoride ions at pH 7.2 - 9.0 and 30 °C by a factor of ten billion (1010).	+	* '''PROBLEM''': The membrane presents a significant permeability barrier for the organophosphates. Alternatives have been used by chemical engineers by immobilizing the enzymes on the cell surface and attaching the cells to a cellulose membrane to use in a bioreactor. Can we ... get around that? I guess we can't do much about this unless we can.

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Latest revision as of 02:27, 4 August 2007

AustinDay 21:14, 2 August 2007 (EDT)

• Chris said that if we can find a transporter that will pump in the toxin, that it might still work. I've started to spend a bit of time looking for some sort of organophosphate transporter in a bacterium. But I'll concentrate on the alcohol side trip more for now, I think it gets across the point that we can detox stuff using this e. coli chassis, and it avoids using a transporter.

AustinDay 02:21, 31 July 2007 (EDT)

• All nerve agents belong to the organophosphorus (OP) compound group.
• They inhibit acetocholinesterase, resulting in the buildup of acetocholine, which prevents muscles from relaxing.
• This other dude (I have all the refs on my computer, the wiki isn't very good for this) Has identified various phosphotriesterases that will detoxify a variety of organophosphates (pesticides, as well as the chemical weapons sarin and soman). He describes various mutants and their effects on the stereoselectivity.
• I've heard of two things used to detox:
  • Phosphotriesterase
  • organophosphorus hydrolase (OPH)

• PROBLEM: The membrane presents a significant permeability barrier for the organophosphates. Alternatives have been used by chemical engineers by immobilizing the enzymes on the cell surface and attaching the cells to a cellulose membrane to use in a bioreactor. Can we ... get around that? I guess we can't do much about this unless we can.