Melbourne/Blue Photosensor Background
From 2007.igem.org
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====Preliminaries==== | ====Preliminaries==== | ||
| - | SopII - sensory rhodopsin II | + | == |
| + | SopII == | ||
| + | - sensory rhodopsin II | ||
[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | ||
[[Melbourne/Blue Photosensor Background/SopII/AA|AA]] | [[Melbourne/Blue Photosensor Background/SopII/AA|AA]] | ||
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| - | HtrII - sensory rhodopsin II transducer | + | |
| + | == HtrII == | ||
| + | - sensory rhodopsin II transducer | ||
[[Melbourne/Blue Photosensor Background/HtrIIA|DNA]], | [[Melbourne/Blue Photosensor Background/HtrIIA|DNA]], | ||
[[Melbourne/Blue Photosensor Background/HtrII/AA|AA]] | [[Melbourne/Blue Photosensor Background/HtrII/AA|AA]] | ||
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| - | SopII/HtrII fusion | + | == |
| + | SopII/HtrII fusion == | ||
| + | |||
[[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA]], | ||
[[Melbourne/Blue Photosensor Background/Fusion/AA|AA]] (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA | [[Melbourne/Blue Photosensor Background/Fusion/AA|AA]] (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA | ||
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| - | ComP - two-component sensor histidine kinase | + | |
| + | == ComP == | ||
| + | - two-component sensor histidine kinase | ||
[[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, | [[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, | ||
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]] | [[Melbourne/Blue Photosensor Background/ComP/AA|AA]] | ||
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| - | ComA - sensory rhodopsin II transducer | + | |
| + | == ComA == | ||
| + | - sensory rhodopsin II transducer | ||
[[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]], | ||
[[Melbourne/Blue Photosensor Background/ComA/AA|AA]] | [[Melbourne/Blue Photosensor Background/ComA/AA|AA]] | ||
Revision as of 11:37, 6 August 2007
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Contents |
Preliminaries
== SopII ==
- sensory rhodopsin II
DNA, AA [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- HaeII (@168) present
HtrII
- sensory rhodopsin II transducer
DNA, AA [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3702851&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum genbank]
- No IGEM restriction sites
- No useful restriction site
==
SopII/HtrII fusion ==
DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP
- two-component sensor histidine kinase
DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] SpeI (@927) deleted HaeII (@1902) inserted
ComA
- sensory rhodopsin II transducer
DNA, AA [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] SpeI (@12) deleted HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
