Template:BerkiGEM2007 vinniSequencingFiles
From 2007.igem.org
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| - | | | + | |otsAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Great no problems with forward until after 900bp. Insert was completely present.||[[BerkiGEM2007-Sequencing-otsAF | otsAF]] |
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| + | |otsAR||06/29/07 ||pBca9145-Bca1144||#2||go1001||Full insert with only problem is a 6 bp sequence after the XhoI site that I heard others have had problems with. Insert was completely present.||[[BerkiGEM2007-Sequencing-otsAR | otsAR]] | ||
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| + | |otsBF||06/29/07 ||pBca9145-Bca1144||#3||ca998||The forward primer completely sequenced the insert except for one point mutation that was after the stop codon and before the BamHI site. Insert was completely present.||[[BerkiGEM2007-Sequencing-otsBF | otsBF]] | ||
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| + | |otsBR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Took care of the one point mutation. Insert was completely present.||[[BerkiGEM2007-Sequencing-otsBR | otsBR]] | ||
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| + | |otsBAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Covers most of otsB and covers point mutation. Insert was not completely present.||[[BerkiGEM2007-Sequencing-otsBAF | otsBAF]] | ||
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| + | |otsBAAF||06/29/07 ||pBca9145-Bca1144||#2||otsAF||Covers the middle of otsA to the end. Insert was not completely present.||[[BerkiGEM2007-Sequencing-otsBAAF | otsBAAF]] | ||
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| + | |otsBABF||06/29/07 ||pBca9145-Bca1144||#3||otsBF||Covers from middle of otsB to middle of otsA. Insert was not completely present.||[[BerkiGEM2007-Sequencing-otsBABF | otsBABF]] | ||
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| + | |otsBAR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Covers from the end to almost beginning of otsA. Insert was not completely present.||[[BerkiGEM2007-Sequencing-otsBAR | otsBAR]] | ||
Latest revision as of 18:18, 7 August 2007
|otsAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Great no problems with forward until after 900bp. Insert was completely present.|| otsAF
|otsAR||06/29/07 ||pBca9145-Bca1144||#2||go1001||Full insert with only problem is a 6 bp sequence after the XhoI site that I heard others have had problems with. Insert was completely present.|| otsAR
|otsBF||06/29/07 ||pBca9145-Bca1144||#3||ca998||The forward primer completely sequenced the insert except for one point mutation that was after the stop codon and before the BamHI site. Insert was completely present.|| otsBF
|otsBR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Took care of the one point mutation. Insert was completely present.|| otsBR
|otsBAF||06/29/07 ||pBca9145-Bca1144||#1||ca998||Covers most of otsB and covers point mutation. Insert was not completely present.|| otsBAF
|otsBAAF||06/29/07 ||pBca9145-Bca1144||#2||otsAF||Covers the middle of otsA to the end. Insert was not completely present.|| otsBAAF
|otsBABF||06/29/07 ||pBca9145-Bca1144||#3||otsBF||Covers from middle of otsB to middle of otsA. Insert was not completely present.|| otsBABF
|otsBAR||06/29/07 ||pBca9145-Bca1144||#4||go1001||Covers from the end to almost beginning of otsA. Insert was not completely present.|| otsBAR
